单细胞分析初探：数据获取入门指南

共有两名患者：

• 患者2586-4： IDDescriptionGSM3330559Tumor Disc PreGSM3330560Tumor Disc ARGSM3330561PBMC PreGSM3330562PBMC Disc EarlyGSM3330563PBMC Disc RespGSM3330564PBMC Disc AR
  • The primary patient (2586-4) received hypofractionated radiation for HLA upregulation to some but not all disease sites
  • 利用10X 3' Chromium v2.0平台建库 + Hiseq2500 "rapid run"模式 GSE117988
  • discovery tumor部分：After sequence alignment and filtering, 7431 tumor cells (2243 cells before and 5188 cells after T cell therapy）
  • discovery PBMC部分：After sequence alignment and filtering, a total of 12,874 cells were analyzed [其中包含了四个时间点：治疗前(Pre)，治疗后早期day +27(Early)，治疗后反应期day+37(Resp)，治疗后复发+614 (AR)]
• 患者9245-3： IDDescriptionGSM3317833PBMC Relapse - L001GSM3317834PBMC Relapse - L002GSM3317835Tumor Relapse - L001GSM3317836Tumor Relapse - L002
  • The second validation patient (9245-3) is a 59-year-old man with metastatic MCC that had initially presented as stage IIIB disease, now metastatic at multiple sites
  • 利用10X 5' V(D)J 进行cell washing, barcoding and library prep+ NovaSeq 6000(gene expression) + Hiseq4000 (V(D)J) GSE118056