signature=44f53d3915b88c9e7b3b8713e10c1307,Stromal Cell Signature Associated with Response to Neoadj...

Figure 1

Unsupervised hierarchical clustering of stromal cells microdissected from tumors categorized according to estrogen receptor status (determined by immunohistochemistry of FFPE tumor fragment and shown in the upper panel). Estrogen receptor (ER) positive marked in green. The ER and progesterone receptor (PR) expression in malignant cells were evaluated using anti-estrogen receptor alpha rabbit monoclonal antibody SP1 (Thermo Fisher Scientific, Walthan, MA, USA) and CONFIRM antiprogesterone receptor rabbit monoclonal antibody (Roche AB, Christian Sundberg, Stockholm, Sweden), respectively, and were considered positive if ≥1% malignant cells were stained. Stromal cells were microdissected from samples. The gene expression profile was determined using Agilent platform and 51 sequences were found differentially expressed. Each column represents one tumor sample and each line represents the expression of one sequence (green less expressed and red, more expressed). Gene symbol appears on the right. Lines on the top of the dendogram show: black, blue, green indicate high confidence; yellow and pink indicate low confidence (color scale in accordance to support is represented in the box, on the right). Characteristics of patients and tumor samples appear on the upper box: outcome (blue, alive with disease recurrence and black, deceased); age (red, ≤40y); ER immunohistochemistry (green, positive); DS (downstaging) (pink, yes). Green (more expressed) and red (less expressed) scale bar on the top.